rv
eil
lance
- View Article
eillance - View Article
" href="http://ismsie55.engagingbeautifulstrippedfemalepows.com/feed//id/abdelkarim-harouni/map.html" />rveillance - View Article
| F. Allerberger1, L. Beutin2,
T. Cheasty3, G. Conedera4 , H. Karch5 (1) Federal Public Health Laboratory, Innsbruck, Austria (2) Robert Koch Institute, Berlin, Germany (3) Istituto Zooprofilattico Sperimentale delle Venezie, Cordenons, Italy (4) PHLS Laboratory of Enteric Pathogens, London, United Kingdom |
| In 1997 the Austrian Federal Ministry of Health
established a reference laboratory for enterohaemorrhagic Escherichia coli at the
federal public health laboratory in Innsbruck. The reference laboratory investigates
sources of infection causing food poisoning in order to prevent further illness, in
collaboration, when appropriate, with the European Union (EU) project ‘Network
for Epidemiological Surveillance and Control of Communicable Diseases in the
Community’. A specific example of such European cooperation follows. A 3 year old boy with mucous haemorrhagic diarrhoea but without fever was admitted to the paediatric ward of a Viennese hospital on 4 April 1999. A stool specimen obtained the same day yielded enterohaemorrhagic Escherichia coli (EHEC) O157. The child remained in hospital for seven days and recovered without complications. The incubation time of EHEC infections is usually three to eight days (1). From 29 March until 4 April 1999, the child had been on holiday with his family in northern Italy and had drunk raw milk at a neighbouring farm. Stool specimens taken from milking cows at this farm, within the framework of the EU project mentioned above, were negative, but EHEC O157 was isolated from the stool specimen of a calf. We did not search for cases among other people who drank raw milk from this farm. Subtyping of EHEC isolates from the child and the calf using XbaI pulsed field gel electrophoresis, phage typing, and Shiga toxin typing – methods described in detail elsewhere (2) – showed that these two strains (laboratory no. EH16 and EH27) were indistinguishable. Five other EHEC O157 strains were isolated from other patients in Austria in the first five months of 1999 (table 1); these were clearly distinguishable from the two strains by the combination of phage type, Shiga toxin, and PFGE patterns (table 2). Table 1: Origin of EHEC O157 isolates and clinical manifestations
|
Isolate No. |
Patient : age, sex, town and state |
Date of stool specimen |
Clinical symptoms |
In/out patient |
EH 9 |
76 years, female, Wels, UA |
7 March 1999 |
Diarrhoea |
Inpatient |
EH 10 |
11 months, male, Linz, UA |
15 March 1999 |
Diarrhoea |
Outpatient |
EH 11 |
10 months, female, Vienna, V |
22 March 1999 |
Hemorrhagic diarrhoea and HUS |
Inpatient
|
EH 16 |
3 years, male, Vienna, V |
04 April 1999 |
Hemorrhagic diarrhoea |
Inpatient
tIsmsie55 Engagingbeautifulstrippedfemalepows Id Abdelkarim Harouni Map Engaging Beautiful Stripped Female Pows
Eurosu |